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PKC alpha 抗体 (N-Term)

Name: PKC alpha 抗体 (N-Term)
SKU: ABIN652517
Availability: OutOfStock

PKCa 适用: 人, 小鼠 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB20769 unconjugated

Western blot analysis of PRKCA Antibody (N-term) (ABIN652517 and ABIN2842346) in mouse NIH-3T3 cell line lysates (35 μg/lane). (PKC alpha 抗体 (N-Term))

PRKCA Antibody (N-term) (ABIN652517 and ABIN2842346) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). (PKC alpha 抗体 (N-Term))

FACS

Confocal immunofluorescent analysis of PRKCA Antibody (N-term) (ABIN652517 and ABIN2842346) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PKC alpha 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PKC alpha 抗体 (N-Term))

IHC (p)

4 images

产品编号 ABIN652517

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
23

23

16

9

8

7

6

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 75-103, N-Term
适用
141

71

49

5

5

3

3

2

2

2

1

1

1
人, 小鼠
宿主
139

11

1
兔
Compatible Secondaries
克隆类型
117

33
多克隆
标记
81

11

9

5

4

4

4

4

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
109

59

46

19

17

17

16

15

14

7

6

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

预测反应

B, Rb, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This PRKCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human PRKCA.

克隆位点

RB20769

亚型

Ig Fraction

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anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
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anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
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anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody
PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PRKCA (PKCa 产品)

背景

PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

分子量

76750

基因ID

5578

NCBI登录号

NP_002728

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling