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His Tag 抗体

Name: His Tag 抗体
SKU: ABIN652278
Availability: OutOfStock

适用: 人 WB 宿主: 兔 Polyclonal RB19960 unconjugated

All lanes : Anti-His Tag Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: 293T/17 transfected with 12tag whole cell lysate Lane 3: 12tag recombinant protein Lysates/proteins at 1 μg per lane. (His Tag 抗体)

Anti-His Tag at 1:8000 dilution + 12tag recombination protein Lysates/proteins at 20 ng per lane. (His Tag 抗体)

2 images

(1 reference)

产品编号 ABIN652278

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag
适用
20

5

4

1

1

1
人
宿主
91

59

11

9

5

4

1

1
兔
Compatible Secondaries
克隆类型
93

75

10
多克隆
标记
78

21

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
应用范围
140

91

39

27

24

18

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB)

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

Poly-HIS peptide were used to produced this antibody.

克隆位点

RB19960

亚型

Ig Fraction

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anti-His Tag antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FLISA, FM 宿主: 小鼠 Monoclonal 33D10-D2 DyLight 649

应用备注

WB: 1:8000. WB: 1:1000. WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Jiang, Zhao, Xiong, Wang, He, Wang, Chen, Geng, Huang, Ouyang, Lai: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." in: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).
抗原

His Tag

别名

6 X His Tag (His Tag 产品)

物质类

Tag

背景

Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.