This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the C-terminal region of human UBE2H.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2H is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100 % identical to the mouse homolog and 98 % identical to the frog and zebrafish homologs, which indicates that this enzyme is highly conserved in eukaryotes.