Optimal conditions to be detrmined by user. Indirect: To detect hNAP-2 byIndirect ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech's Biotinylated Anti-Human NAP-2 (500-P03GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. To detect hNAP-2 by WB analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
浓度
Lot specific
缓冲液
Lyophilized from PBS, pH 7.2.
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
抗原
NAP1L4
(Nucleosome Assembly Protein 1-Like 4 (NAP1L4))
NAP-2 is a CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is produced in leukocytes by enzymatic processing of a precursor called platelet basic protein (PBP). NAP-2 chemoattracts and activates neutrophils.