RFC5 抗体
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- 抗原 See all RFC5 抗体
- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
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适用
- 人, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RFC5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 纯化方法
- Affinity purified
- 免疫原
- RFC5 antibody was raised using a synthetic peptide corresponding to a region with amino acids METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINE
- Top Product
- Discover our top product RFC5 Primary Antibody
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- 应用备注
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WB: 1 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator. - 说明
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RFC5 Blocking Peptide, catalog no. 33R-5977, is also available for use as a blocking control in assays to test for specificity of this RFC5 antibody
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of RFC5 antibody in PBS
- 浓度
- Lot specific
- 缓冲液
- PBS
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- 抗原
- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
- 别名
- RFC5 (RFC5 产品)
- 别名
- zgc:110313 antibody, 2610020K06Rik antibody, 2610209F07Rik antibody, 36.5kDa antibody, 36kDa antibody, Recc5 antibody, RFC36 antibody, replication factor C (activator 1) 5 antibody, replication factor C subunit 5 antibody, replication factor C subunit 5 L homeolog antibody, rfc5 antibody, Rfc5 antibody, RFC5 antibody, rfc5.L antibody
- 背景
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC5 is the 36 kDa subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
- 分子量
- 38 kDa (MW of target protein)
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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