Mitochondrial Marker 抗体
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- 抗原
- Mitochondrial Marker
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 原理
- Mouse anti-Human Mitochondria Marker Antibody [Sodium Azide Free]
- 特异性
- Mitochondria in cytoplasm
- 纯化方法
- The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
- 免疫原
- The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
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- 应用备注
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FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 缓冲液
- In 1X PBS ( pH 7.4), BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- 抗原
- Mitochondrial Marker
- 别名
- Mitochondria Marker
- 背景
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Target Description: MAb MTC754 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC754 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals including insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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