Nucleolin 抗体
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- 抗原 See all Nucleolin (NCL) 抗体
- Nucleolin (NCL)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nucleolin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Mouse anti-Human NCL Antibody [Sodium Azide Free]
- 特异性
- Nucleoli
- 纯化方法
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- 免疫原
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- Top Product
- Discover our top product NCL Primary Antibody
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 缓冲液
- In 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- 抗原
- Nucleolin (NCL)
- 别名
- Nucleolin (NCL 产品)
- 别名
- C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
- 背景
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Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Gene Symbol: NCL
- 基因ID
- 4691
- UniProt
- P19338
- 途径
- Ribonucleoprotein Complex Subunit Organization
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