IGLV1-51 抗体
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- 抗原 See all IGLV1-51 products
- IGLV1-51 (Immunoglobulin lambda Variable 1-51 (IGLV1-51))
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IGLV1-51 antibody is un-conjugated
- 应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Mouse anti-Human Lambda Light Chain Antibody [Sodium Azide Free]
- 特异性
- Cell Surface, Cytoplasmic and Secreted
- 纯化方法
- IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.
- 免疫原
- IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT - 限制
- 仅限研究用
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- 缓冲液
- In 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- 抗原
- IGLV1-51 (Immunoglobulin lambda Variable 1-51 (IGLV1-51))
- 别名
- Immunoglobulin lambda variable 1-51 (IGLV1-51 产品)
- 别名
- IGLV151 antibody, V1-19 antibody, immunoglobulin lambda variable 1-51 antibody, IGLV1-51 antibody
- 背景
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Target Description: Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 3:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed). Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]
Gene Symbol: IGLV1-51///IGLC1
- 基因ID
- 3535
- UniProt
- P01701, P0CG04
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