Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
原理
AMPKα1/2 is a protein encoded by the PRKAA1 gene which is approximately 64 kDa. AMPKα1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, mTOR signalling, AMP-activated protein kinase signalling, the regulation of TP53 activity and longevity regulating pathway. It is a catalytic subunit of AMP-activated protein kinase, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, it activates energy-producing pathways and inhibits energy-consuming processes such as protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPKα1/2 is expressed in nervous system, liver, blood, intestine and kidney. Mutations in the PRKAA1 gene may result in obesity. STJ91581 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of AMPKα1/2 protein.
特异性
AMPKα1/2 Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein.
产品特性
Rabbit polyclonal to AMPKα1/2.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
Synthesized peptide derived from human AMPKalpha1/2 around the non-phosphorylation site of T183/172.