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PRMT5 抗体 (Internal Region)

Name: PRMT5 抗体 (Internal Region)
SKU: ABIN6266359
Availability: OutOfStock

PRMT5 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of NIH-3T3 cell lysate, using PRMT5 Antibody. (PRMT5 抗体 (Internal Region))

ABIN6276704 staining HeLa cells by ICC/IF. (PRMT5 抗体 (Internal Region))

IF/ICC

ABIN6276704 at 1/100 staining Mouse lung tissue by IHC-P. (PRMT5 抗体 (Internal Region))

IHC

Western blot analysis of extracts from rat brain, using PRMT5 Antibody. (PRMT5 抗体 (Internal Region))

4 images

产品编号 ABIN6266359

发货至: 中国

Datasheet as PDF

抗原 See all PRMT5 抗体

PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
抗原表位
15

11

9

8

6

5

4

3

3

2

2

1

1

1

1

1

1
Internal Region
适用
88

36

18

8

6

6

3

3

3

2

2

2

2
人, 小鼠, 大鼠
宿主
80

6

1

1
兔
Compatible Secondaries
克隆类型
72

15

1
多克隆
标记
46

5

5

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This PRMT5 antibody is un-conjugated
应用范围
59

32

32

14

13

13

10

7

4

3

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

PRMT5 Antibody detects endogenous levels of total PRMT5.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human PRMT5, corresponding to a region within the internal amino acids.

亚型

IgG

Top Product

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anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 40-140) antibody
PRMT5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 21-70) antibody
PRMT5 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, 猴, 豚鼠, Bat, Pig, 斑马鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) (N-Term) antibody
PRMT5 适用: 人, 小鼠, 大鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 297-534) antibody
PRMT5 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) antibody
PRMT5 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 2H4 unconjugated Recombinant Antibody

anti-Protein Arginine Methyltransferase 5 (PRMT5) (N-Term) antibody
PRMT5 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, Cow, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) antibody
PRMT5 适用: 人, 小鼠 WB, IHC, IF, IP, ChIP, IC 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 76-104) antibody
PRMT5 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 591-621) antibody
PRMT5 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Protein Arginine Methyltransferase 5 (PRMT5) antibody
PRMT5 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

别名

PRMT5 (PRMT5 产品)

别名

HRMT1L5 antibody, IBP72 antibody, JBP1 antibody, SKB1 antibody, SKB1Hs antibody, Jbp1 antibody, Skb1 antibody, si:zc14a17.2 antibody, skb1 antibody, wu:fb95f10 antibody, zgc:110669 antibody, hsl7 antibody, DDBDRAFT_0187422 antibody, DDBDRAFT_0235403 antibody, DDB_0187422 antibody, DDB_0235403 antibody, NV18830 antibody, jbp1 antibody, ibp72 antibody, skb1hs antibody, hrmt1l5 antibody, protein arginine methyltransferase 5 antibody, protein arginine N-methyltransferase 5 antibody, protein arginine methyltransferase 5 L homeolog antibody, putative arginine N-methyltransferase, type II antibody, Skb1 family protein antibody, Protein arginine N-methyltransferase 5 antibody, PRMT5 antibody, Prmt5 antibody, prmt5 antibody, prmt5.L antibody, LOC100115766 antibody, prmt-5 antibody

背景

Description: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PubMed:10531356, PubMed:11152681, PubMed:11747828, PubMed:12411503, PubMed:15737618, PubMed:17709427, PubMed:20159986, PubMed:20810653, PubMed:21258366, PubMed:21917714, PubMed:22269951, PubMed:21081503). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), such methylation being required for the assembly and biogenesis of snRNP core particles (PubMed:12411503, PubMed:11747828, PubMed:17709427). Methylates SUPT5H and may regulate its transcriptional elongation properties (PubMed:12718890). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity). Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR, this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PubMed:21917714, PubMed:21258366). Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PubMed:21917714). Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PubMed:22269951). Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PubMed:20810653). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter (By similarity). Methylates GM130/GOLGA2, regulating Golgi ribbon formation (PubMed:20421892). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789). Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805). Along with LYAR, binds the promoter of gamma-globin HBG1/HBG2 and represses its expression (PubMed:25092918). Symmetrically methylates NCL (PubMed:21081503).
Gene: PRMT5

分子量

73kDa

基因ID

10419

UniProt

O14744

途径

Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development