GSK3 beta 抗体 (N-Term)
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- 抗原 See all GSK3 beta (GSK3b) 抗体
- GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))
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抗原表位
- N-Term
- 适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GSK3 beta antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 特异性
- GSK3 alpha/ beta Antibody detects endogenous levels of total GSK3 alpha/ beta.
- 预测反应
- Pig,Zebrafish,Rabbit,Dog,Xenopus
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human GSK3 alpha/ beta, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product GSK3b Primary Antibody
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anti-Glycogen Synthase Kinase 3 beta (GSK3b) antibody
GSK3b 适用: 人, 小鼠 WB, IF, IP 宿主: 小鼠 Monoclonal 2E6-D6-C12 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 4-34), (N-Term) antibodyGSK3b 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03816 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 4-34) antibodyGSK3b 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 30-59) antibodyGSK3b 适用: 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 362-395) antibodyGSK3b 适用: 大鼠 WB 宿主: 兔 Polyclonal RB56577 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 2-33) antibodyGSK3b 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB56641 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) (AA 2-33), (pSer9) antibodyGSK3b 适用: 人 WB 宿主: 兔 Polyclonal RB56641 unconjugated
anti-Glycogen Synthase Kinase 3 beta (GSK3b) antibodyGSK3b 适用: 人, 小鼠, 大鼠 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal 2E6-D6-C12 unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))
- 别名
- GSK3B (GSK3b 产品)
- 别名
- GSK3 antibody, GSK-3 antibody, C86142 antibody, GSK-3beta antibody, 7330414F15Rik antibody, 8430431H08Rik antibody, GSK3beta antibody, fk80d11 antibody, GSK-3[b] antibody, wu:fb68h05 antibody, wu:fk80d11 antibody, gsk-3 antibody, gsk3 antibody, gsk3-beta antibody, gsk3beta antibody, xgsk-3 antibody, glycogen synthase kinase 3 beta antibody, glycogen synthase kinase 3 beta L homeolog antibody, GSK3B antibody, Gsk3b antibody, gsk3b antibody, gsk3b.L antibody
- 背景
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Description: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation. Phosphorylates SFPQ at 'Thr-687' upon T-cell activation. Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2. Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation. Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation. Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Phosphorylates MYCN in neuroblastoma cells which may promote its degradation (PubMed:24391509).
Gene: GSK3B
- 分子量
- 48kDa
- 基因ID
- 2932
- UniProt
- P49841
- 途径
- WNT signaling, Hedgehog Signaling, Fc-epsilon Receptor Signaling Pathway, Cellular Glucan Metabolic Process, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Autophagy, BCR Signaling, Warburg Effect
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