TUT1 抗体 (N-Term)
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- 抗原 See all TUT1 抗体
- TUT1 (Terminal Uridylyl Transferase 1, U6 SnRNA-Specific (TUT1))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TUT1 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- TUTase Antibody detects endogenous levels of total TUTase.
- 预测反应
- Bovine,Horse,Rabbit,Dog
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human TUTase, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product TUT1 Primary Antibody
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- 应用备注
- WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- TUT1 (Terminal Uridylyl Transferase 1, U6 SnRNA-Specific (TUT1))
- 别名
- TUT1 (TUT1 产品)
- 别名
- PAPD2 antibody, RBM21 antibody, STARPAP antibody, TUTase antibody, 2700038E08Rik antibody, Rbm21 antibody, zgc:112254 antibody, terminal uridylyl transferase 1, U6 snRNA-specific antibody, TUT1 antibody, Tut1 antibody, tut1 antibody
- 背景
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Description: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
Gene: TUT1
- 分子量
- 95kDa
- 基因ID
- 64852
- UniProt
- Q9H6E5
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