-
- 抗原 See all TOP1MT 抗体
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
-
抗原表位
- Internal Region
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This TOP1MT antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA
- 特异性
- TOP1MT Antibody detects endogenous levels of total TOP1MT.
- 预测反应
- Pig,Zebrafish,Horse,Sheep,Chicken
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human TOP1MT, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product TOP1MT Primary Antibody
-
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibody
TOP1MT 适用: 人 IF, IHC, WB, FACS, StM 宿主: 小鼠 Monoclonal TOP1MT-488 unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal TOP1MT-568 unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 470-550) antibodyTOP1MT 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 IHC, StM 宿主: 兔 Monoclonal TOP1MT-2883R unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 270-400) antibodyTOP1MT 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 11-238) antibodyTOP1MT 适用: 小鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 1-238) antibodyTOP1MT 适用: 大鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
-
- 抗原
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- 别名
- TOP1MT (TOP1MT 产品)
- 别名
- 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
- 背景
-
Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Gene: TOP1MT
- 分子量
- 70 kDa
- 基因ID
- 116447
- UniProt
- Q969P6
-
You are here:
