T-Bet 抗体 (Internal Region)
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- 抗原 See all T-Bet 抗体
- T-Bet
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This T-Bet antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Tbx21 Antibody detects endogenous levels of total tbx21.
- 预测反应
- Pig,Bovine,Sheep,Rabbit,Dog
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human tbx21, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product T-Bet Primary Antibody
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anti-T-Bet (AA 241-493) antibody
适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 338-387) antibody适用: 人, 大鼠, Cow, 犬, Pig, 兔, 豚鼠, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (Middle Region) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-T-Bet antibody适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 160-399) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 326-535) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 387-486) antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F8 unconjugated
anti-T-Bet (AA 400-535) antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F10 unconjugated
anti-T-Bet (AA 82-395) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (C-Term) antibody适用: 人, 小鼠 WB, ELISA, IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:1000-3000, IHC 1:200, IF1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- T-Bet
- 别名
- TBX21 (T-Bet 产品)
- 别名
- T-PET antibody, T-bet antibody, TBET antibody, TBLYM antibody, TBT1 antibody, Tbet antibody, Tblym antibody, T-box 21 antibody, TBX21 antibody, Tbx21 antibody
- 背景
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Description: Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses, controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity).
Gene: TBX21
- 分子量
- 58 kDa
- 基因ID
- 30009
- UniProt
- Q9UL17
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