Reelin 抗体 (N-Term)
(1 validation)-
- 抗原 See all Reelin (RELN) 抗体
- Reelin (RELN)
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抗原表位
- N-Term
- 适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Reelin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 特异性
- RELN Antibody detects endogenous levels of total RELN.
- 预测反应
- Pig,Horse,Sheep,Rabbit,Dog,Chicken
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human RELN, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product RELN Primary Antibody
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anti-Reelin (RELN) (AA 3143-3461) antibody
RELN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 26-190) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C8 unconjugated
anti-Reelin (RELN) (AA 2978-3373) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Reelin (RELN) (AA 26-254) antibodyRELN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) antibodyMicroarray verified RELN 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB299520 unconjugated
anti-Reelin (RELN) (AA 26-190) antibodyRELN 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 2086-2296) antibodyRELN 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 3142-3460) antibodyRELN 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 2400-2610) antibodyRELN 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 2400-2610) antibodyRELN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C16 unconjugated
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- 应用备注
- WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104518
- 日期
- 2024.01.26
- 抗原
- Reelin
- Lot Number
- 10M5728
- Method validated
- Immunohistochemistry
- Positive Control
Postnatal day 5 and adult (> 2 months) CD1 mouse brain fixed in 4% paraformaldehyde.
- Negative Control
One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.
- Notes
Passed. The Reelin antibody (AA 491-590) ABIN6264693 works in IHC-P at 1:50 concentrations with microwave antigen retrieval.
- Primary Antibody
- ABIN6264693
- Secondary Antibody
- anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A11037, lot # 2439060)
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix of samples in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Cut brain with a microtome into 6 µm sections and mount on glass slides.
- After paraffin removal processed sections for microwave antigen retrieval for 10 minutes (95-100° C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, wash sections (distilled water, 2 times for 5 minutes each; followed by PBS for 5 minutes).
- Incubate sections for 1 h at RT in a humid chamber in PBS containing 1% ovalbumin (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary rabbit anti-Reelin (antibodies-online, ABIN6264693, lot 10M5728) diluted 1:50, in PBS-BSA-PLL ON at RT in a humid chamber.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A11037, lot # 2439060), 1:500, in 0.1M PBS, 1 hour at room temperature.
- Wash sections 3x 5 min with 0.01 M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
- Experimental Notes
生效 #104518 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Reelin expression in the postnatal day 5 mouse cerebellum. EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex;PCL/ML = Purkinje cell/molecular layers of the cerebellar cortex; WM = white matter. Staining is mainly observed in the processes of the pre-migratory granule cells. Original magnifications: A=10x; B= 20x]()
Reelin expression in the postnatal day 5 mouse cerebellum. EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex;PCL/ML = Purkinje cell/molecular layers of the cerebellar cortex; WM = white matter. Staining is mainly observed in the processes of the pre-migratory granule cells. Original magnifications: A=10x; B= 20x
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- Reelin (RELN)
- 别名
- RELN (RELN 产品)
- 别名
- LIS2 antibody, PRO1598 antibody, RL antibody, reeler antibody, rl antibody, Reelen antibody, Rl antibody, reln antibody, RELN antibody, MGC172539 antibody, reelin antibody, reelin L homeolog antibody, RELN antibody, Reln antibody, reln antibody, reln.L antibody, LOC579060 antibody
- 背景
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Description: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation (By similarity).
Gene: RELN
- 分子量
- 388kDa
- 基因ID
- 5649
- UniProt
- P78509
- 途径
- Synaptic Membrane
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