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PML 抗体 (N-Term)

Name: PML 抗体 (N-Term)
SKU: ABIN6264315
Availability: OutOfStock

PML 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of HeLa lysate using PML antibody. (PML 抗体 (N-Term))

ABIN6276587 at 1/100 staining Human kidney tissue by IHC-P. (PML 抗体 (N-Term))

IHC

IF/ICC

F6318 staining HEPG2 cells by ICC/IF. (PML 抗体 (N-Term))

IF/ICC

4 images

(1 reference)

产品编号 ABIN6264315

发货至: 中国

Datasheet as PDF

抗原 See all PML 抗体

PML (Promyelocytic Leukemia (PML))
抗原表位
8

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
56

9

6

2

2

2

1

1

1
人, 小鼠, 大鼠
宿主
49

10
兔
Compatible Secondaries
克隆类型
49

10
多克隆
标记
43

4

3

1

1

1

1

1

1

1

1

1
This PML antibody is un-conjugated
应用范围
50

26

17

16

9

7

7

5

3

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

特异性

PML Antibody detects endogenous levels of total PML.

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human PML, corresponding to a region within N-terminal amino acids.

亚型

IgG

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应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
Wu, Shao, Zhou, Sun, Wang, Yan, Liu, Wu, Ye, Huang, Zhou et al.: "Autophagy and Ubiquitin-Mediated Proteolytic Degradation of PML/Rarα Fusion Protein in Matrine-Induced Differentiation Sensitivity Recovery of ATRA-Resistant APL (NB4-LR1) Cells: in Vitro and in Vivo ..." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 48, Issue 6, pp. 2286-2301, (2018) (PubMed).
抗原

PML (Promyelocytic Leukemia (PML))

别名

PML (PML 产品)

别名

MYL antibody, PP8675 antibody, RNF71 antibody, TRIM19 antibody, 1200009E24Rik antibody, AI661194 antibody, Trim19 antibody, RGD1562602 antibody, PML antibody, promyelocytic leukemia antibody, PML antibody, Pml antibody

背景

Description: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Isoform PML-4 has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Isoform PML-4 also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, regulates double-strand break repair in gamma-irradiation-induced DNA damage responses via its interaction with WRN, acts as a negative regulator of telomerase by interacting with TERT, and regulates PER2 nuclear localization and circadian function. Isoform PML-6 inhibits specifically the activity of the tetrameric form of PKM. The nuclear isoforms (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5) in concert with SATB1 are involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Isoform PML-2 is required for efficient IFN-gamma induced MHC II gene transcription via regulation of CIITA. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. PML also regulates transcription activity of ELF4 and can act as an important mediator for TNF-alpha- and IFN-alpha-mediated inhibition of endothelial cell network formation and migration.
Gene: PML

分子量

98kDa

基因ID

5371

UniProt

P29590

途径

p53 Pathway, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus