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PARP1 抗体 (Internal Region)

Name: PARP1 抗体 (Internal Region)
SKU: ABIN6264040
Availability: OutOfStock

PARP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Hela whole cell lysates, using PARP1 Antibody. (PARP1 抗体 (Internal Region))

ABIN6277453 at 1/100 staining Rat brain tissue by IHC-P. (PARP1 抗体 (Internal Region))

IHC

2 images

(1 reference)

产品编号 ABIN6264040

发货至: 中国

Datasheet as PDF

抗原 See all PARP1 抗体

PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
抗原表位
16

15

13

9

8

8

8

8

8

8

8

7

7

7

7

7

6

5

5

4

4

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
206

51

45

8

6

6

6

6

5

4

4

2

2

1

1
人, 小鼠, 大鼠
宿主
199

24

1
兔
Compatible Secondaries
克隆类型
188

36
多克隆
标记
134

16

15

13

12

12

2

2

2

2

2

2

2

2

2

2

2
This PARP1 antibody is un-conjugated
应用范围
146

121

69

33

33

27

24

22

21

17

16

7

7

3

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

PARP1 Antibody detects endogenous levels of total PARP1.

预测反应

Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human PARP1, corresponding to a region within the internal amino acids.

亚型

IgG

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anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 661-881) antibody
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anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) antibody
PARP1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3G4 unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (C-Term) antibody
PARP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 670-858) antibody
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anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody
PARP1 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Monoclonal 8C7 unconjugated Recombinant Antibody

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (cleaved) antibody
PARP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 183-214) antibody
PARP1 适用: 人 WB, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." in: Marine drugs, Vol. 16, Issue 3, (2018) (PubMed).
抗原

PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

别名

PARP1 (PARP1 产品)

背景

Description: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1, HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
Gene: PARP1

分子量

113kDa

基因ID

142

UniProt

P09874

途径

Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location