我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

OGT 抗体 (N-Term)

Name: OGT 抗体 (N-Term)
SKU: ABIN6263805
Availability: OutOfStock

OGT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Hela whole cell lysates, using OGT Antibody. (OGT 抗体 (N-Term))

ABIN6277003 at 1/100 staining Mouse liver tissue by IHC-P. (OGT 抗体 (N-Term))

IHC

2 images

产品编号 ABIN6263805

发货至: 中国

Datasheet as PDF

抗原 See all OGT 抗体

OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))
抗原表位
5

4

4

2

2

1

1

1

1

1

1

1
N-Term
适用
55

30

27

2

2

2

2

2

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
53

2

1
兔
Compatible Secondaries
克隆类型
44

12
多克隆
标记
31

3

3

3

3

3

3

3

3

1
This OGT antibody is un-conjugated
应用范围
32

15

14

11

10

7

5

3

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

特异性

OGT Antibody detects endogenous levels of total OGT.

预测反应

Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human OGT, corresponding to a region within N-terminal amino acids.

亚型

IgG

Top Product

Discover our top product OGT Primary Antibody
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (Internal Region) antibody
Verified OGT 适用: 人, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 857-1036) antibody
OGT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, IF, IHC (p), IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 606-1022) antibody
OGT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 8G7 unconjugated Recombinant Antibody

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

别名

OGT (OGT 产品)

别名

BcDNA:GH04245 antibody, CG10392 antibody, Dmel\\CG10392 antibody, OGT antibody, Ogt antibody, P1201 antibody, SXC antibody, Sxc antibody, l(2)02637 antibody, l(2)NC130 antibody, sxc/Ogt antibody, fm81g08 antibody, ogt antibody, wu:fc12b01 antibody, wu:fm81g08 antibody, HRNT1 antibody, O-GLCNAC antibody, 1110038P24Rik antibody, 4831420N21Rik antibody, AI115525 antibody, Ogtl antibody, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog antibody, super sex combs antibody, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 antibody, O-linked N-acetylglucosamine (GlcNAc) transferase antibody, O-linked GlcNAc transferase antibody, o-linked GlcNAc transferase antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, ogt.L antibody, sxc antibody, ogt.1 antibody, OGT antibody, ogt antibody, GL50803_12081 antibody, GL50803_41701 antibody, THAPSDRAFT_264441 antibody, spyA antibody, Ogt antibody

背景

Description: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc) (PubMed:26678539). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1 (PubMed:26678539). Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (PubMed:26678539). Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination (PubMed:26678539). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed:22121020, PubMed:23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed:24474760). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:12150998, PubMed:18288188, PubMed:19377461, PubMed:19451179, PubMed:20018868, PubMed:20200153, PubMed:21285374, PubMed:15361863). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (PubMed:21285374, PubMed:28584052, PubMed:28302723).
Gene: OGT

分子量

117kDa

基因ID

8473

UniProt

O15294

途径

Regulation of Carbohydrate Metabolic Process