LIG4 抗体 (C-Term)
Quick Overview for LIG4 抗体 (C-Term) (ABIN6262967)
抗原
See all LIG4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- DNA Ligase IV Antibody detects endogenous levels of total DNA Ligase IV.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human DNA Ligase IV, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))
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别名
- LIG4
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背景
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Description: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Gene: LIG4
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分子量
- 104kDa
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基因ID
- 3981
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UniProt
- P49917
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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