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HSP70 1A 抗体 (C-Term)

Name: HSP70 1A 抗体 (C-Term)
SKU: ABIN6262398
Availability: OutOfStock

HSPA1A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of HSP70 expression in Hela cells (HSP70 1A 抗体 (C-Term))

ABIN6269012 at 1/100 staining Rat heart tissue by IHC-P. (HSP70 1A 抗体 (C-Term))

IHC

IF/ICC

3 images

(2 references)

产品编号 ABIN6262398

发货至: 中国

Datasheet as PDF

抗原 See all HSP70 1A (HSPA1A) 抗体

HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
抗原表位
12

11

7

6

6

5

5

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
112

32

25

20

15

11

10

9

7

6

6

6

5

4

4

4

3

3

3

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
84

46
兔
Compatible Secondaries
克隆类型
84

46
多克隆
标记
67

16

14

6

6

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HSP70 1A antibody is un-conjugated
应用范围
111

50

45

35

29

16

16

10

8

5

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

HSP70 Antibody detects endogenous levels of total HSP70.

预测反应

Pig,Bovine,Sheep,Xenopus

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human HSP70, corresponding to a region within C-terminal amino acids.

亚型

IgG

Top Product

Discover our top product HSPA1A Primary Antibody
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (C-Term) antibody
HSPA1A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (Center) antibody
HSPA1A 适用: 人, 小鼠, 大鼠 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 285-641) antibody
HSPA1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibody
HSPA1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) antibody
HSPA1A 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3C6 unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 500-600) antibody
HSPA1A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 285-641) antibody
HSPA1A 适用: 人 WB 宿主: 小鼠 Monoclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibody
HSPA1A 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibody
HSPA1A 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2# unconjugated

anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 418-512) antibody
HSPA1A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
Xian, Gong, Li, Guo, Jiang: "Exosomes with Highly Angiogenic Potential for Possible Use in Pulp Regeneration." in: Journal of endodontics, Vol. 44, Issue 5, pp. 751-758, (2018) (PubMed).

Shan, Wang, Xu, Wang, Cao, Liu, Zhu: "Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid." in: Journal of Alzheimer's disease : JAD, Vol. 49, Issue 3, pp. 829-44, (2016) (PubMed).
抗原

HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

别名

HSPA1A (HSPA1A 产品)

别名

hsp70-4 antibody, hspa1a antibody, Hsp70-3 antibody, Hsp70.3 antibody, Hsp72 antibody, hsp68 antibody, hsp70A1 antibody, HSP72 antibody, Hsp70-1 antibody, Hspa1 antibody, Hspa1b antibody, hsp70-1 antibody, hsp70-1a antibody, hsp70i antibody, hsp72 antibody, hspa1 antibody, hspa1b antibody, HSP70-1 antibody, HSP70-1A antibody, HSP70I antibody, HSPA1 antibody, HSPA1A antibody, HSPA1B antibody, HSP70-2 antibody, HSPA2 antibody, HSP70 antibody, hspA1A antibody, heat shock cognate 70-kd protein, tandem duplicate 3 antibody, heat shock 70 kDa protein 1 antibody, heat shock 70 kDa protein II antibody, heat shock protein 1A antibody, heat shock 70kD protein 1A antibody, heat shock protein family A (Hsp70) member 1A S homeolog antibody, heat shock protein family A (Hsp70) member 1A antibody, heat shock protein 70.2 antibody, heat shock 70kDa protein 1A antibody, heat shock 70 kDa protein 1B antibody, heat shock protein 70.1 antibody, hsp70.3 antibody, LOC100023597 antibody, LOC100554002 antibody, LOC100608888 antibody, Hspa1a antibody, hspa1a.S antibody, HSPA1A antibody, HSP70.2 antibody, LOC100354037 antibody, HSP70.1 antibody

背景

Description: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1 (PubMed:24012426, PubMed:26865365, PubMed:24318877). Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation (PubMed:27708256). Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle (PubMed:27137183). Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling (PubMed:24613385). Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223). Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401).
Gene: HSPA1A

分子量

70 kDa

基因ID

3303, 3304

UniProt

P0DMV8

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process