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ELF1 抗体 (C-Term)

Name: ELF1 抗体 (C-Term)
SKU: ABIN6261511
Availability: OutOfStock
Rating: 5 (1 reviews)

ELF1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody (ABIN6261511) (ELF1 抗体 (C-Term))

Western blot analysis on 293 cell lysate using ELF1 Antibody (ELF1 抗体 (C-Term))

IF/ICC

4 images

(1 validation)

产品编号 ABIN6261511

发货至: 中国

Datasheet as PDF

抗原 See all ELF1 抗体

ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
抗原表位
15

4

4

4

2

1

1

1

1

1

1

1
C-Term
适用
44

7

7

6

4

3

2

2

1

1

1

1
人
宿主
40

3

1
兔
Compatible Secondaries
克隆类型
43

1
多克隆
标记
24

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ELF1 antibody is un-conjugated
应用范围
35

13

13

11

10

6

5

3

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

ELF1 Antibody detects endogenous levels of total ELF1.

预测反应

Horse

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human ELF1, corresponding to a region within C-terminal amino acids.

亚型

IgG

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应用备注

WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
生效 #104221 (Western Blotting)

by

Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine

No.

#104221

日期

2019.10.28

抗原

ELF1

Lot Number

4318850

Method validated

Western Blotting

Positive Control

A549 (endogenous ELF1), ELF1 recombinant protein

Negative Control

Notes

Passed. ABIN6261511 recognizes recombinant and endogenous human ELF1. The antibody does also reveal an additional larger MW protein band in A549 cell lysates.

Validation Images

Detection of human ELF1 by western blot using ABIN6261511 diluted 1:1000. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 67kDa.
Full Methods
Primary Antibody

ABIN6261511

Secondary Antibody

goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234)

Full Protocol

Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO₂ to 3x10⁴ cells/cm² in 2ml on a 6 well plate (Costar, 3516).
Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
Take cells up in growth medium.
Wash cells once with 1x PBS.
Resuspend approximately 2.05x10⁵cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
Denature samples for 3min at 95°C and subsequently keep them on ice.
Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
Incubation with primary
rabbit anti-ELF1 antibody (antibodies-online, ABIN6261511, 4318850) diluted 1:1000 in TBST containing 0.5% skim milk ON at 4°C.
loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
Wash membrane 3x for 10min with TBST.
Incubation with secondary
goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
Wash membrane 3x for 10min with TBST.
Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).

Experimental Notes

ABIN6261511 reveals a protein with an apparent molecular weight of approximately 62kDa. The expected molecular weight for endogenous human ELF1 is approximately 67kDa. The antibody also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).
Other ELF1-antibody dilutions were tested, but a dilution of 1:2500 was found optimal in terms of minimal background and strength of signal.
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))

别名

ELF1 (ELF1 产品)

别名

zef1 antibody, fb05d07 antibody, wu:fb05d07 antibody, Elf-1 antibody, Sts1 antibody, mElf-1 antibody, p70 antibody, xelf1 antibody, elf-1 antibody, E74 like ETS transcription factor 1 antibody, E74-like ETS transcription factor 1 antibody, E74-like factor 1 antibody, E74 like ETS transcription factor 1 L homeolog antibody, E74 like ETS transcription factor 1 S homeolog antibody, ELF1 antibody, elf1 antibody, Elf1 antibody, elf1.L antibody, elf1.S antibody

背景

Description: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
Gene: ELF1

分子量

67kDa

基因ID

1997

UniProt

P32519