DNA2 抗体 (Internal Region)
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- 抗原 See all DNA2 抗体
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DNA2 antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- DNA2L Antibody detects endogenous levels of total DNA2L.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human DNA2L, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product DNA2 Primary Antibody
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anti-DNA Replication Helicase 2 Homolog (DNA2) (AA 1-300) antibody
DNA2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Replication Helicase 2 Homolog (DNA2) antibodyDNA2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Replication Helicase 2 Homolog (DNA2) (Middle Region) antibodyDNA2 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
- 别名
- DNA2 (DNA2 产品)
- 别名
- DNA2L antibody, Dna2l antibody, E130315B21Rik antibody, PEOA6 antibody, hDNA2 antibody, XDna2 antibody, dna2-A antibody, DNA replication helicase/nuclease 2 antibody, DNA replication helicase/nuclease 2 S homeolog antibody, DNA2 antibody, Dna2 antibody, dna2.S antibody
- 背景
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Description: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
Gene: DNA2
- 分子量
- 120 kDa
- 基因ID
- 1763
- UniProt
- P51530
- 途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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