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- 抗原 See all AGO2 抗体
- AGO2 (Argonaute 2 (AGO2))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AGO2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- AGO2 Antibody detects endogenous levels of total AGO2.
- 预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Xenopus
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human AGO2, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product AGO2 Primary Antibody
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anti-Argonaute 2 (AGO2) (AA 1-377) antibody
AGO2 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 2E12-1C9 unconjugated
anti-Argonaute 2 (AGO2) (AA 517-818) antibodyAGO2 适用: 人 WB, ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) antibodyAGO2 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 1-100) antibodyAGO2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 47-115) antibodyAGO2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 27-59), (N-Term) antibodyAGO2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB8013 unconjugated
anti-Argonaute 2 (AGO2) (AA 1-377) antibodyAGO2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 381-480) antibodyAGO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H2 unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- AGO2 (Argonaute 2 (AGO2))
- 别名
- AGO2 (AGO2 产品)
- 别名
- EIF2C2 antibody, T19E23.7 antibody, T19E23_7 antibody, argonaute 2 antibody, Q10 antibody, Eif2c2 antibody, Argonaute2 antibody, eif2c1 antibody, eif2c2 antibody, 1110029L17Rik antibody, 2310051F07Rik antibody, AI225898 antibody, AL022874 antibody, AW546247 antibody, ENSMUSG00000072493 antibody, Gerp95 antibody, Gm10365 antibody, mKIAA4215 antibody, AGO2 antibody, AG02 antibody, AGO 2 antibody, Ago-2 antibody, Ago2 antibody, CG13452 antibody, CG7439 antibody, Dm Ago2 antibody, Dmel\\CG7439 antibody, ago antibody, ago-2 antibody, ago2 antibody, dAGO2 antibody, dAgo2 antibody, eIF2C2 antibody, argonaute 2, RISC catalytic component antibody, Argonaute family protein antibody, argonaute 2, RISC catalytic component L homeolog antibody, argonaute RISC catalytic subunit 2 antibody, argonaute RISC catalytic component 2 antibody, Argonaute 2 antibody, AGO2 antibody, Ago2 antibody, ago2.L antibody, ago2 antibody
- 背景
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Description: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Gene: AGO2
- 分子量
- 90-95 kDa
- 基因ID
- 27161
- UniProt
- Q9UKV8
- 途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
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