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MHC Class II RT-1B 抗体

适用: 大鼠, 小鼠 FACS, IHC (fro), IHC (p), IHC, WB 宿主: 小鼠 Monoclonal OX-6 unconjugated
产品编号 ABIN625901
发货至: 中国
  • 抗原 See all MHC Class II RT-1B products
    MHC Class II RT-1B
    适用
    大鼠, 小鼠
    宿主
    • 8
    小鼠
    克隆类型
    • 8
    单克隆
    标记
    • 2
    • 2
    • 2
    • 2
    This MHC Class II RT-1B antibody is un-conjugated
    应用范围
    • 8
    • 3
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
    品牌
    IHC-plus™
    特异性
    Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.
    纯化方法
    Protein G purified
    免疫原
    Rat thymocyte membrane glycoprotein.

    Type of Immunogen: Membrane extract
    克隆位点
    OX-6
    亚型
    IgG1
  • 应用备注
    Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

    Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer  pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)
    说明

    Target Species of Antibody: Rat

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.2, 0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    MHC Class II RT-1B
    Abstract
    MHC Class II RT-1B 产品
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