USP28 抗体 (N-Term)
Quick Overview for USP28 抗体 (N-Term) (ABIN6258349)
抗原
See all USP28 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- USP28 Antibody detects endogenous levels of total USP28.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human USP28, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Ubiquitin Specific Peptidase 28 (USP28) (AA 430-580) antibody
USP28 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 28 (USP28) (AA 430-580) antibodyUSP28 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 28 (USP28) (AA 1-583) antibodyUSP28 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 28 (USP28) (Internal Region) antibodyVerified USP28 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 28 (USP28) (C-Term) antibodyUSP28 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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有效期
- 12 months
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- USP28 (Ubiquitin Specific Peptidase 28 (USP28))
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别名
- USP28
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背景
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Description: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).
Gene: USP28
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分子量
- 122 kDa
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基因ID
- 57646
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UniProt
- Q96RU2
抗原
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