SIRT1 抗体 (AA 577-590)
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- 抗原 See all SIRT1 抗体
- SIRT1 (Sirtuin 1 (SIRT1))
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抗原表位
- AA 577-590
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SIRT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 原理
- Sirtuin 1 / SIRT1 (aa577-590)
- 序列
- EKPQEVQTSR NVES
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_036370.2, NP_001135970.1).
- 交叉反应
- 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2, NP_001135970.1.
- 亚型
- IgG
- Top Product
- Discover our top product SIRT1 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5 μg/mL.
Western Blot: Approx 120 kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7 kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Ac
Peptide ELISA: antibody detection limit dilution 1:64000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- SIRT1 (Sirtuin 1 (SIRT1))
- 别名
- SIRT1 (SIRT1 产品)
- 别名
- sirtuin 1 antibody, AA673258 antibody, SIR2L1 antibody, Sir2 antibody, Sir2a antibody, Sir2alpha antibody, sir2l1 antibody, sirtuin antibody, SIR2 antibody, sirtuin 1 antibody, sirtuin 1 L homeolog antibody, SIRT1 antibody, SRT1 antibody, Sirt1 antibody, sirt1.L antibody
- 背景
- SIRT1, sirtuin 1, SIR2L1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, sir2-like 1, sirtuin type 1
- 基因ID
- 23411, 93759
- NCBI登录号
- NP_036370, NP_001135970
- 途径
- MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
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