SDF4 抗体 (AA 161-175)
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- 抗原 See all SDF4 抗体
- SDF4 (Stromal Cell Derived Factor 4 (SDF4))
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抗原表位
- AA 161-175
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SDF4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- SDF4 (aa161-175)
- 序列
- EYKVKFLASK GHSEK
- 特异性
- This antibody is expected to recognize both reported isoforms NP_057631.2, and NP_057260.3
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- CEYKVKFLASKGHSEK
- 亚型
- IgG
- Top Product
- Discover our top product SDF4 Primary Antibody
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10 μg/mL.
Western Blot: Approx 45 kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8 kDa according to NP_057260.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- SDF4 (Stromal Cell Derived Factor 4 (SDF4))
- 别名
- SDF4 (SDF4 产品)
- 别名
- Cab45 antibody, SDF-4 antibody, MGC107861 antibody, wu:fb66d06 antibody, wu:fd46e11 antibody, zgc:56577 antibody, zgc:76916 antibody, CAB45 antibody, stromal cell derived factor 4 antibody, stromal cell derived factor 4 L homeolog antibody, SDF4 antibody, sdf4 antibody, sdf4.L antibody, Sdf4 antibody
- 背景
- SDF4, stromal cell derived factor 4, Cab45, 45 kDa calcium-binding protein, OTTHUMP00000001754, SDF-4, calcium binding protein, stromal cell-derived factor 4
- 基因ID
- 51150, 20318, 155173
- NCBI登录号
- NP_057631, NP_057260
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