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- 抗原 See all POLA1 抗体
- POLA1 (DNA Polymerase alpha (POLA1))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- DNA Polymerase alpha Antibody detects endogenous levels of total DNA Polymerase alpha.
- 预测反应
- Pig,Horse
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human DNA Polymerase alpha, corresponding to a region within N-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product POLA1 Primary Antibody
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anti-DNA Polymerase alpha (POLA1) (AA 1363-1462) antibody
POLA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1406-1439) antibodyPOLA1 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB57637 unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1240-1462) antibodyPOLA1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 310-832) antibodyPOLA1 适用: Thermus aquaticus WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1-33) antibodyPOLA1 适用: 人 WB 宿主: 兔 Polyclonal RB57636 unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1363-1462) antibodyPOLA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1209-1465) antibodyPOLA1 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- POLA1 (DNA Polymerase alpha (POLA1))
- 别名
- POLA1 (POLA1 产品)
- 别名
- pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody
- 背景
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Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).
Gene: POLA1
- 分子量
- 165 kDa
- 基因ID
- 5422
- UniProt
- P09884
- 途径
- SARS-CoV-2 Protein Interactome
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