RPL5 抗体 (C-Term)
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- 抗原 See all RPL5 抗体
- RPL5 (Ribosomal Protein L5 (RPL5))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RPL5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- RPL5 Antibody detects endogenous levels of total RPL5.
- 预测反应
- Bovine,Horse,Dog,Chicken,Xenopus
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human RPL5, corresponding to a region within C-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product RPL5 Primary Antibody
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- 应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- RPL5 (Ribosomal Protein L5 (RPL5))
- 别名
- RPL5 (RPL5 产品)
- 别名
- DBA6 antibody, L5 antibody, U21RNA antibody, CG17489 antibody, Dmel\\CG17489 antibody, E-2d antibody, M(2)40B antibody, Rp L5 antibody, Rpl5 antibody, dRPL5 antibody, yip6 antibody, rpl5 antibody, wu:fj02g05 antibody, zgc:86854 antibody, mg:cb01f08 antibody, rpl5l antibody, zgc:56511 antibody, zgc:77176 antibody, ribosomal protein L5 antibody, 60S ribosomal protein L5 antibody, Ribosomal protein L5 antibody, ribosomal protein L5 L homeolog antibody, 50S ribosomal protein L5 antibody, ribosomal protein L5 S homeolog antibody, ribosomal protein L5b antibody, rpl5 antibody, ribosomal protein L5a antibody, Rpl5 antibody, RPL5 antibody, rpl-5 antibody, RpL5 antibody, rpl5.L antibody, rpl5 antibody, rpl5.S antibody, rpl5b antibody, rpl5a antibody
- 背景
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Description: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:24120868, PubMed:23636399). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868).
Gene: RPL5
- 分子量
- 34 kDa
- 基因ID
- 6125
- UniProt
- P46777
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