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FAK 抗体 (pTyr397)

Name: FAK 抗体 (pTyr397)
SKU: ABIN6256730
Availability: OutOfStock

PTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Phospho-FAK (Tyr397) expression in various lysates (FAK 抗体 (pTyr397))

ABIN6267607 at 1/200 staining Human lung cancer tissue sections by IHC-P. (FAK 抗体 (pTyr397))

IHC

IF/ICC

ABIN6267607 at 1/200 staining Human heart tissue sections by IHC-P. (FAK 抗体 (pTyr397))

IHC

Western blot analysis of FAK phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (FAK 抗体 (pTyr397))

IF/ICC

8 images

(3 references)

产品编号 ABIN6256730

发货至: 中国

Datasheet as PDF

抗原 See all FAK (PTK2) 抗体

FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
抗原表位
43

35

33

29

28

22

22

18

16

16

11

11

8

7

6

6

5

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
pTyr397
适用
350

304

231

21

21

17

16

11

8

6

6

4

3

3

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
362

17

3

2

1

1
兔
Compatible Secondaries
克隆类型
358

28
多克隆
标记
166

19

17

13

10

10

10

10

10

10

10

10

10

10

10

10

8

8

8

8

8

8

2

1
This FAK antibody is un-conjugated
应用范围
301

134

133

121

119

70

66

44

31

27

21

4

2

2
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 397.

预测反应

Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.

亚型

IgG
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 700-800) antibody
PTK2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 798-1041) antibody
PTK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 355-490) antibody
PTK2 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 2C3 unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr576) antibody
PTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 396-423) antibody
PTK2 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20775 unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 1-680) antibody
PTK2 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr861) antibody
PTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
PTK2 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 10H7 unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
PTK2 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 10H7E9 unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (C-Term) antibody
PTK2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
Zhang, Zhou, Meng, Zhang, Zhou: "Down-regulating Myoferlin inhibits the vasculogenic mimicry of melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition." in: Journal of cellular and molecular medicine, Vol. 22, Issue 3, pp. 1743-1754, (2018) (PubMed).

Huang, Song, Tao, Shao, Zeng, Xu, Qi, Sun: "Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195610, (2018) (PubMed).

Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).
抗原

FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

别名

PTK2 (PTK2 产品)

别名

FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody

背景

Description: Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation, required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation, this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC, this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1, most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1, GIT2 and SHC1, this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Gene: PTK2

分子量

119kDa

基因ID

5747

UniProt

Q05397

途径

Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling