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NPM1 抗体 (pThr199)

NPM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Phospho-NPM (Thr199) expression in various lysates (NPM1 抗体 (pThr199))

ABIN6267323 at 1/200 staining Human lung cancer tissue sections by IHC-P. (NPM1 抗体 (pThr199))

ABIN6267323 at 1/200 staining Mouse testis tissue sections by IHC-P. (NPM1 抗体 (pThr199))

ABIN6267323 staining HT-29 cells by ICC/IF. (NPM1 抗体 (pThr199))

ABIN6267323 at 1/200 staining Mouse liver tissue sections by IHC-P. (NPM1 抗体 (pThr199))

ABIN6267323 staining Hela by IF/ICC. (NPM1 抗体 (pThr199))

Western blot analysis of NPM phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (NPM1 抗体 (pThr199))

ABIN6267323 at 1/200 staining Mouse kidney tissue sections by IHC-P. (NPM1 抗体 (pThr199))

8 images

产品编号 ABIN6256499

发货至: 中国

Datasheet as PDF

抗原 See all NPM1 抗体

NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
抗原表位
25

15

15

13

9

8

8

7

7

5

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr199
适用
153

109

74

13

12

10

9

9

7

3

3

2

1

1
人, 小鼠, 大鼠
宿主
145

30

1

1
兔
Compatible Secondaries
克隆类型
145

33
多克隆
标记
100

13

5

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3
This NPM1 antibody is un-conjugated
应用范围
123

61

49

39

28

28

27

14

12

11

7

7

7

5

5

4

3

3

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-Nucleophosmin (Thr199) Antibody detects endogenous levels of Nucleophosmin only when phosphorylated at Threonine 199.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human Nucleophosmin around the phosphorylation site of Thr199.

亚型

IgG

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anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 1-294) antibody
NPM1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B2 unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibody
NPM1 适用: 人 WB, IF, IHC (p), IP, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 20-154) antibody
NPM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 212-292) antibody
NPM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibody
NPM1 适用: 人 WB, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal NPM1-3286 unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibody
NPM1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rNPM1-1901 unconjugated Recombinant Antibody

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibody
NPM1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal NPM1-1902 unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) antibody
Verified NPM1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 7-33) antibody
NPM1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 198-226) antibody
NPM1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

别名

NPM1 (NPM1 产品)

别名

B23 antibody, NPM antibody, NO38 antibody, Npm antibody, B23NP antibody, npm4 antibody, npm antibody, CG7917 antibody, CRP1 antibody, Crp1 antibody, Dmel\\CG7917 antibody, dNLP antibody, dNLP-S antibody, p22 antibody, npm1 antibody, sb:cb487 antibody, nucleophosmin 1 antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog antibody, nucleophosmin 1b antibody, Nucleophosmin antibody, nucleophosmin antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) antibody, Nucleoplasmin antibody, nucleophosmin 1a antibody, NPM1 antibody, Npm1 antibody, npm1.L antibody, npm1b antibody, npm antibody, Nlp antibody, npm1a antibody

背景

Description: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Gene: NPM1

分子量

32/38kDa

基因ID

4869

UniProt

P06748

途径

Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly

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