MED1 抗体 (pThr1457)
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- 抗原 See all MED1 抗体
- MED1 (Mediator Complex Subunit 1 (MED1))
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抗原表位
- pThr1457
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MED1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.
- 预测反应
- Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- 纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- 免疫原
- A synthesized peptide derived from human TRAP220/MED1 around the phosphorylation site of Thr1457.
- 亚型
- IgG
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anti-Mediator Complex Subunit 1 (MED1) (AA 1391-1490) antibody
MED1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1-212) antibodyMED1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 878-1031) antibodyMED1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1-300) antibodyMED1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (Center) antibodyMED1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (pThr1457) antibodyMED1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) antibodyMED1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (Internal Region) antibodyMED1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- MED1 (Mediator Complex Subunit 1 (MED1))
- 别名
- MED1 (MED1 产品)
- 背景
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Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
Gene: MED1
- 分子量
- 168kDa
- 基因ID
- 5469
- UniProt
- Q15648
- 途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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