我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

MED1 抗体 (pThr1457)

Name: MED1 抗体 (pThr1457)
SKU: ABIN6256479
Availability: OutOfStock

MED1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Phospho-PPAR-BP (Thr1457) expression in various lysates (MED1 抗体 (pThr1457))

IF/ICC

Western blot analysis of PPAR-BP phosphorylation expression in Serum treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (MED1 抗体 (pThr1457))

ABIN6267655 at 1/100 staining human Breast carcinoma tissue sections by IHC-P. (MED1 抗体 (pThr1457))

IHC

ABIN6267655 staining HeLa cells by ICC/IF. (MED1 抗体 (pThr1457))

IF/ICC

5 images

产品编号 ABIN6256479

发货至: 中国

Datasheet as PDF

抗原 See all MED1 抗体

MED1 (Mediator Complex Subunit 1 (MED1))
抗原表位
15

7

6

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr1457
适用
63

35

13

4

2

2

2

2

2
人, 小鼠
宿主
57

4

2

1
兔
Compatible Secondaries
克隆类型
59

5
多克隆
标记
32

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This MED1 antibody is un-conjugated
应用范围
28

23

13

13

12

12

9

7

3

3

3

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.

预测反应

Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human TRAP220/MED1 around the phosphorylation site of Thr1457.

亚型

IgG
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibody
MED1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (AA 1391-1490) antibody
MED1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A2 unconjugated

anti-Mediator Complex Subunit 1 (MED1) (AA 1-212) antibody
MED1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (AA 878-1031) antibody
MED1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibody
MED1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (AA 1-300) antibody
MED1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (Center) antibody
MED1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (pThr1457) antibody
MED1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) antibody
MED1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Mediator Complex Subunit 1 (MED1) (Thr2647) antibody
MED1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

MED1 (Mediator Complex Subunit 1 (MED1))

别名

MED1 (MED1 产品)

别名

CRSP1 antibody, CRSP200 antibody, DRIP205 antibody, DRIP230 antibody, PBP antibody, PPARBP antibody, PPARGBP antibody, RB18A antibody, TRAP220 antibody, TRIP2 antibody, CRSP-1 antibody, MED1 antibody, pbp antibody, nbr1 antibody, crsp1 antibody, rb18a antibody, trip2 antibody, pparbp antibody, crsp200 antibody, drip205 antibody, drip230 antibody, ppargbp antibody, trap220 antibody, CG7162 antibody, Dmel\\CG7162 antibody, Med1 antibody, Med1/SOP3/TRAP220 antibody, Trap220 antibody, dTRAP220 antibody, AI480703 antibody, CRSP210 antibody, Pparbp antibody, l11Jus15 antibody, RGD1559552 antibody, mediator complex subunit 1 antibody, calcitonin-related polypeptide beta antibody, Mediator complex subunit 1 antibody, mediator of RNA polymerase II transcription subunit 1 antibody, MED1 antibody, CALCB antibody, med1 antibody, Med1 antibody

背景

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
Gene: MED1

分子量

168kDa

基因ID

5469

UniProt

Q15648

途径

Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha