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CHEK1 抗体 (pSer296)

CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Chk1 phosphorylation expression in UV treated HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining mouse brain tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 staining HeLa by IF/ICC. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining human lung cancer tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining rat lung tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining mouse testicular tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining human Cholangio-carcinoma tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining human skin tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining mouse gastric tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining rat heart tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining human glioma tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

ABIN6267221 at 1/100 staining rat testicular tissue sections by IHC-P. (CHEK1 抗体 (pSer296))

12 images

产品编号 ABIN6256251

发货至: 中国

Datasheet as PDF

抗原 See all CHEK1 抗体

CHEK1 (Checkpoint Kinase 1 (CHEK1))
抗原表位
36

26

26

15

13

9

7

6

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer296
适用
195

117

67

16

11

9

6

6

6

4

4

3

2

1

1
人, 小鼠, 大鼠
宿主
205

14

6

5
兔
Compatible Secondaries
克隆类型
207

24
多克隆
标记
123

13

9

8

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

3

3

3

3
This CHEK1 antibody is un-conjugated
应用范围
165

87

52

41

40

36

31

14

11

10

7

6

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-Chk1 (Ser296) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 296.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human Chk1 around the phosphorylation site of Ser296.

亚型

IgG

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anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody
CHEK1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 适用: 人, 小鼠, 大鼠, 犬, 马, 兔, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 小鼠, 小鸡 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-Checkpoint Kinase 1 (CHEK1) antibody
CHEK1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 2G1D5 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 人 WB, IHC (p), DB 宿主: 兔 Polyclonal RB7845-RB18425 unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
CHEK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

CHEK1 (Checkpoint Kinase 1 (CHEK1))

别名

CHEK1 (CHEK1 产品)

别名

An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

背景

Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.
Gene: CHEK1

分子量

45kDa

基因ID

1111

UniProt

O14757

途径

p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair

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