CD247 抗体 (pTyr142)
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- 抗原 See all CD247 抗体
- CD247 (CD247 Molecule (CD247))
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抗原表位
- pTyr142
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD247 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Phospho-CD3 zeta (Tyr142) Antibody detects endogenous levels of CD3 zeta only when phosphorylated at Tyr142.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit
- 纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- 免疫原
- A synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142.
- 亚型
- IgG
- Top Product
- Discover our top product CD247 Primary Antibody
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anti-CD247 Molecule (CD247) (Tyr72) antibody
CD247 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal EM-26 unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 88-153) antibodyCD247 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 50-164) antibodyCD247 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 ELISA, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 WB, FACS, IHC 宿主: 兔 Monoclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-165) antibodyCD247 适用: 人 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 51-100) antibodyCD247 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (pTyr111), (pTyr123) antibodyCD247 适用: 人, 小鼠 WB, FACS 宿主: 小鼠 Monoclonal EM-55 unconjugated
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- 应用备注
- WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- CD247 (CD247 Molecule (CD247))
- 别名
- CD247 (CD247 产品)
- 背景
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Description: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Gene: CD247
- 分子量
- 19 kDa
- 基因ID
- 919
- UniProt
- P20963
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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