LIMK-1/2 (pThr505), (pThr508) 抗体
-
- 抗原
- LIMK-1/2
-
抗原表位
- pThr505, pThr508
-
适用
- 人, 大鼠, 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Phospho-LIMK1/2 (Thr508/505) Antibody detects endogenous levels of LIMK1/2 only when phosphorylated at Threonine 508/505
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- 免疫原
- A synthesized peptide derived from human LIMK1/2 around the phosphorylation site of Threonine 508/505
- 亚型
- IgG
-
anti-LIMK-1/2 (Ser104) antibody
适用: 人, 大鼠, 小鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-LIMK-1/2 (C-Term), (pSer505), (pSer508) antibody适用: 人, 大鼠, 小鸡, Cow WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-LIMK-1/2 (pThr505), (pThr508) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-LIMK-1/2 (AA 481-530) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-LIMK-1/2 (C-Term) antibody适用: 人, 大鼠, 小鼠, 小鸡, Cow WB, IP 宿主: 兔 Polyclonal unconjugated
anti-LIMK-1/2 (C-Term), (pSer505), (pSer508) antibody适用: 人, 大鼠, 小鼠, 小鸡, Cow WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
- 有效期
- 12 months
-
- 抗原
- LIMK-1/2
- 别名
- LIMK1/2
- 背景
-
Description: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
Gene: LIMK1
- 分子量
- 72kDa
- 基因ID
- 3984
- UniProt
- P53667
-
