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NF-kB p65 抗体 (pSer536)

NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of NF kappaB p65 phosphorylation expression in IL-1 treated Raw264. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining mouse kidney tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining mouse testis tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining human brain tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 staining HeLa by IF/ICC. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining rat uterine tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining rat lung tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining mouse gastric tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining human heart tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining human liver tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 at 1/100 staining rat ovarian tissue sections by IHC-P. (NF-kB p65 抗体 (pSer536))

ABIN6267070 staining lovo cells by ICC/IF. (NF-kB p65 抗体 (pSer536))

12 images

(11 references)

产品编号 ABIN6255402

发货至: 中国

Datasheet as PDF

抗原 See all NF-kB p65 (NFkBP65) 抗体

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
抗原表位
32

31

28

26

26

25

24

22

17

17

16

15

15

11

10

9

9

8

5

5

5

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

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2

2

2

2
pSer536
适用
521

371

302

42

39

28

26

20

18

15

14

11

5

2

1

1
人, 小鼠, 大鼠
宿主
481

42

3

2

1
兔
Compatible Secondaries
克隆类型
466

63
多克隆
标记
274

29

21

17

12

10

10

10

10

10

10

10

10

10

10

10

10

9

9

9

9

9

7

2

2
This NF-kB p65 antibody is un-conjugated
应用范围
413

195

134

119

104

98

95

70

67

26

25

9

7

7

7

6

4

3

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

Phospho-NF- kappaB p65 (Ser536) Antibody detects endogenous levels of NF- kappaB p65 only when phosphorylated at Serine 536

交叉反应

人, 小鼠, 大鼠

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Serine 536

亚型

IgG

Top Product

Discover our top product NFkBP65 Primary Antibody
anti-Nuclear Factor-kB p65 (NFkBP65) (AA 51-100) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (Center) antibody
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anti-Nuclear Factor-kB p65 (NFkBP65) (C-Term) antibody
NFkBP65 适用: 人 WB, CP 宿主: 小鼠 Monoclonal 27F9-G4 unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 19-306) antibody
NFkBP65 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (acLys314), (acLys315) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (acLys310) antibody
NFkBP65 适用: 人, 小鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000 IHC 1:50-1:500 IP 1:100-1:500 IF 1:200

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, sodium azide and glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C.Stable for 12 months from date of receipt

有效期

12 months
Zhang, Fei, Tao, Li, Shen, Wang, Liu, Xu: "Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3848595, (2018) (PubMed).

Huang, Zhang, Hou, Wang, Liu, Zhang, Chen, Zhu: "LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 194, (2018) (PubMed).

Ding, Shen, Li, Jiang, Shen: "Therapeutic mild hypothermia improves early outcomes in rats subjected to severe sepsis." in: Life sciences, Vol. 199, pp. 1-9, (2018) (PubMed).

Wu, Zhu, Zhang, Yin, Kang, Cao, Tian, Lu, Liu: "EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7875-7885, (2018) (PubMed).

Li, Zhao, Lin, Gong, An: "TREM2 inhibits inflammatory responses in mouse microglia by suppressing the PI3K/NF-κB signaling." in: Cell biology international, (2018) (PubMed).

Zhu, Xu, Lv, He, Ye, Wang, Hu: "miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways." in: Inflammation, Vol. 41, Issue 4, pp. 1524-1535, (2018) (PubMed).

Wu, Gu, Mei, Xu, Jing, Yao, Chen, Yang, Chen, Yang, Qi, Hu, Wüthrich, Mei: "Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 31, Issue 11, pp. 1826-1834, (2017) (PubMed).

Zhou, Lu, Chen, Ge, Chen, Xu, Shi, Dai, Li, Ju, Cao, Qin, Chen, Teng, Jiang: "AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice." in: Scientific reports, Vol. 7, pp. 43245, (2017) (PubMed).

Zeng, Li, Chen, Lv, Liu, Ran, Sun, Bieerkehazhi, Liu, Li, Lai, Watibieke, Dawulietihan, Li, Li: "Survivin activates NF‑κB p65 via the IKKβ promoter in esophageal squamous cell carcinoma." in: Molecular medicine reports, Vol. 13, Issue 2, pp. 1869-80, (2016) (PubMed).

Jiang, Zheng, Zhang, Yue, Liu, Ma, Yang, Wen: "Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages." in: American journal of translational research, Vol. 8, Issue 11, pp. 4778-4790, (2016) (PubMed).

Yang, Zheng, Zhang, He, Zhang, Ma, Zhang, Wu, Wen: "miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells." in: Biochimica et biophysica acta, Vol. 1852, Issue 7, pp. 1477-89, (2015) (PubMed).
抗原

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

别名

NF kappaB p65 (NFkBP65 产品)

别名

C-Rel antibody, NFKB3 antibody, p65 antibody, NFkB antibody, c-rel antibody, zgc:100833 antibody, Xrel2 antibody, Xrel3 antibody, rel antibody, rel-A antibody, rel2 antibody, rel3 antibody, v-rel antibody, xrel antibody, REL proto-oncogene, NF-kB subunit antibody, RELA proto-oncogene, NF-kB subunit antibody, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, v-rel avian reticuloendotheliosis viral oncogene homolog antibody, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antibody, REL antibody, RELA antibody, Rela antibody, Rel antibody, rel antibody, rel.L antibody

背景

Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).
Gene: RELA

分子量

65kDa

基因ID

5970

UniProt

Q04206

途径

NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins

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