PKM 抗体 (pSer37)
-
- 抗原 See all PKM 抗体
- PKM (Pyruvate Kinase M1/2 (PKM))
-
抗原表位
- pSer37
- 适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This PKM antibody is un-conjugated
-
应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Phospho-PKM2 (Ser37) Antibody detects endogenous levels of PKM2 only when phosphorylated at Ser37.
- 预测反应
- Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus
- 纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- 免疫原
- A synthesized peptide derived from human PKM2 around the phosphorylation site of Ser37.
- 亚型
- IgG
- Top Product
- Discover our top product PKM Primary Antibody
-
-
- 应用备注
- WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
-
- 抗原
- PKM (Pyruvate Kinase M1/2 (PKM))
- 别名
- PKM (PKM 产品)
- 背景
-
Description: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Gene: PKM
- 分子量
- 58 kDa
- 基因ID
- 5315
- UniProt
- P14618
- 途径
- Warburg Effect
-