Igk 抗体
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- 抗原 See all Igk products
- Igk (Immunoglobulin kappa Chain Complex (Igk))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Igk antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human Kappa light chain was used as the immunogen for this Kappa antibody.
- 克隆位点
- KC3L-1
- 亚型
- IgG1 kappa
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anti-Immunoglobulin kappa Chain Complex (Igk) antibody (HRP)
Igk 适用: 小鼠 FACS, ELISA 宿主: 山羊 Polyclonal HRP F(ab')2 fragment
anti-Immunoglobulin kappa Chain Complex (Igk) antibody (FITC)Igk 适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal FITC
anti-Immunoglobulin kappa Chain Complex (Igk) antibody (Alkaline Phosphatase (AP))Igk 适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
anti-Immunoglobulin kappa Chain Complex (Igk) antibody (PE)Igk 适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal PE
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells
WB: 1:250-1:500
IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Kappa antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- Igk (Immunoglobulin kappa Chain Complex (Igk))
- 别名
- Kappa (Igk 产品)
- 别名
- kappa antibody, immunoglobulin kappa chain complex antibody, Igk antibody
- 背景
- This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
- 基因ID
- 3514
- UniProt
- P01601, P01834
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