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P57 抗体

适用: 人, 小鼠 WB, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal KP10 unconjugated
产品编号 ABIN6252521
发货至: 中国
  • 抗原
    P57
    适用
    • 11
    • 7
    • 2
    人, 小鼠
    宿主
    • 7
    • 4
    小鼠
    克隆类型
    • 7
    • 4
    单克隆
    标记
    • 11
    非结合性
    应用范围
    • 9
    • 7
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human protein was used as the immunogen for the p57 antibody.
    克隆位点
    KP10
    亚型
    IgG2b kappa
  • 应用备注
    Western blot: 1-2 μg/mL

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the p57 antibody to be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    P57
    别名
    Clabp antibody, Lmb3 antibody, TACO antibody, p57 antibody, CLABP antibody, CLIPINA antibody, HCORO1 antibody, coronin, actin binding protein 1A antibody, coronin 1A antibody, Coro1a antibody, CORO1A antibody
    背景
    Recognizes a protein of 57  kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.
    基因ID
    1028
    UniProt
    P49918
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