Melanoma 抗体
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- 抗原 See all Melanoma products
- Melanoma
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适用
- 人, 马
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Melanoma antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- 特异性
- Does not react with rat
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
- 克隆位点
- NKI-beteb
- 亚型
- IgG2b kappa
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anti-Melanoma antibody
适用: 人, 犬, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
anti-Melanoma antibody适用: 人, 马 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal NKI-beteb unconjugated
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- Melanoma
- Abstract
- Melanoma 产品
- 背景
- By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
- 基因ID
- 6490
- UniProt
- P40967
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