电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Nuclear Marker 抗体

适用: 人, 小鼠, 大鼠 FACS, IF, ICC, IHC (p), WB 宿主: 小鼠 Monoclonal NM106 unconjugated
产品编号 ABIN6251935
发货至: 中国
  • 抗原 See all Nuclear Marker products
    Nuclear Marker
    适用
    • 5
    • 3
    • 3
    • 2
    人, 小鼠, 大鼠
    宿主
    • 5
    小鼠
    克隆类型
    • 5
    单克隆
    标记
    • 3
    • 2
    This Nuclear Marker antibody is un-conjugated
    应用范围
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    特异性
    Does not react with Pig
    纯化方法
    Protein G affinity chromatography
    免疫原
    Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
    克隆位点
    NM106
    亚型
    IgG1 kappa
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT

    Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Optimal dilution of the Nuclear marker antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    Nuclear Marker
    Abstract
    Nuclear Marker 产品
    背景
    This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
You are here:
客服