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HSP18.5 抗体

HSP18.5-C 适用: Arabidopsis thaliana WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN619791
发货至: 中国
  • 抗原
    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
    适用
    Arabidopsis thaliana
    宿主
    克隆类型
    多克隆
    应用范围
    Western Blotting (WB)
    交叉反应 (详细)
    Not reactive in: no confirmed exceptions from predicted reactivity are currently known
    预测反应
    dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 18.5 kDa
    纯化方法
    serum
    免疫原
    recombinant Arabidopsis thaliana Hsp18.5
  • 应用备注
    1 : 1000 with standard ECL (WB)
    说明

    hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 200 μL of sterile water.
    储存条件
    -20 °C
    储存方法
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • 抗原
    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
    别名
    HSP18.5
    背景
    HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
    分子量
    18.5 kDa
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