Histone 3 抗体
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- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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适用
- Tomato, Arabidopsis thaliana, Zea mays, Chlamydomonas reinhardtii, Moss
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC)
- 预测反应
- dicots including: Vitis vinifera, monocots: Hordeum vulgare, Oryza sativa, Zea mays, trees: Pinus pinaster, algae: Volvox sp.
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 15 / 17 kDa
- 纯化方法
- serum
- 免疫原
- KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At4g10980), H3.2 P59226(At4g09200,At4g27360,At4g10390,At4g10400,At4g65360), H3-like 2 Q9FXI7 (At4g19890)
- Top Product
- Discover our top product H3 Primary Antibody
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- 应用备注
- Recommended Dilution: 1 : 5000 with standard ECL (WB), 1 : 100 - 1 : 500 (ICC)
- 说明
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Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
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Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).
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Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
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- 抗原
- Histone 3 (H3)
- 别名
- H3 (H3 产品)
- 别名
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- 背景
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AGI Code: At4g40030
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4. - 分子量
- expected: 15 kDa, apparent: 17 kDa
- UniProt
- P59169, P59226, Q9FXI7
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