Neurofilament 抗体
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- 抗原 See all Neurofilament products
- Neurofilament
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适用
- 人, 大鼠, 小鼠, 小鸡, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Neurofilament antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-Neurofilament (H+L) (NF421 + NFL736), Purified, with BSA
- 产品特性
- This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)
- 克隆位点
- NF421 NFL-736
- 亚型
- IgG
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anti-Neurofilament antibody
适用: 人, 大鼠, 小鸡, Pig, Cow FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠, 小鸡, Pig FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鸡, Pig, Cow FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠, 小鸡, Pig FACS, IHC (p) 宿主: 小鼠 Monoclonal NF421 unconjugated
anti-Neurofilament (Extracellular Domain) antibody适用: 人 IF, IHC (p) 宿主: 小鼠 Monoclonal SPM563 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠, 小鸡, Pig FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1:25-1:50
- Optimal dilution for a specific application should be determined by user
- 说明
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Brain, Neuroblastoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Neurofilament
- Abstract
- Neurofilament 产品
- 分子量
- 200 kDa & 68 kDa
- 基因ID
- 4744, 198760
- UniProt
- P12036
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