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Neurofilament 抗体 (CF®640R)

适用: 人, 大鼠, 小鼠, Pig, 小鸡 FACS, IHC (f), IF 宿主: 小鼠 Monoclonal NF421 NFL-736 CF®640R
产品编号 ABIN6188742
发货至: 中国
  • 抗原 See all Neurofilament products
    Neurofilament
    适用
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    人, 大鼠, 小鼠, Pig, 小鸡
    宿主
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    小鼠
    克隆类型
    • 21
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    单克隆
    标记
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    This Neurofilament antibody is conjugated to CF®640R
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
    原理
    Mouse Monoclonal anti-Neurofilament (H+L) (NF421 + NFL736), CF640R Conjugate
    产品特性
    This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)
    克隆位点
    NF421 NFL-736
    亚型
    IgG
  • 应用备注
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1:25-1:50
    • Optimal dilution for a specific application should be determined by user
    说明

    Brain, Neuroblastoma

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    Neurofilament
    Abstract
    Neurofilament 产品
    分子量
    200 kDa & 68 kDa
    基因ID
    4744, 198760
    UniProt
    P12036
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