MAML2 抗体 (CF®647)
-
- 抗原 See all MAML2 抗体
- MAML2 (Mastermind-Like 2 (MAML2))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This MAML2 antibody is conjugated to CF®647
-
应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt)
- 原理
- Mouse Monoclonal anti-MAML2 (MAML2/1302), CF647 Conjugate
- 产品特性
- MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J?, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant human full-length MAML2
- 克隆位点
- MAML2-1302
- 亚型
- IgG2a
- Top Product
- Discover our top product MAML2 Primary Antibody
-
-
- 应用备注
-
Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
-
MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
-
- 抗原
- MAML2 (Mastermind-Like 2 (MAML2))
- 别名
- MAML2 (MAML2 产品)
- 别名
- 5930431H10 antibody, BC032967 antibody, MAM-3 antibody, MAM2 antibody, MAM3 antibody, MLL-MAML2 antibody, mastermind like 2 (Drosophila) antibody, mastermind-like transcriptional coactivator 2 antibody, mastermind like transcriptional coactivator 2 antibody, Maml2 antibody, MAML2 antibody
- 背景
- Mam-2, MAML2, Mastermind-like protein 2
- 分子量
- 125 kDa
- 基因ID
- 84441
-