Villin 1 抗体 (AA 179-311)
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- 抗原 See all Villin 1 (VIL1) 抗体
- Villin 1 (VIL1)
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抗原表位
- AA 179-311
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Villin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Mouse Monoclonal anti-Villin (VIL1/1325), Purified, BSA-free
- 产品特性
- This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)
- 克隆位点
- VIL1-1325
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product VIL1 Primary Antibody
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- 应用备注
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Western blotting 1-2 μg/mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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A549, HepG2 and HCT116 cells. Colon or Rectum.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- Villin 1 (VIL1)
- 别名
- Villin (VIL1 产品)
- 别名
- D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
- 背景
- VIL1, Villin-1, Villin1
- 分子量
- 93 kDa
- 基因ID
- 7429, 654595
- UniProt
- P09327
- 途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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