Myosin (Smooth Muscle Heavy Chain) 抗体
适用: 人, 大鼠, 小鸡, 兔, 犬, 豚鼠, Pig, Cat, Cow
FACS, IF, IHC (f)
宿主: 小鼠
Monoclonal
ID8
unconjugated
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- 抗原 See all Myosin (Smooth Muscle Heavy Chain) products
- Myosin (Smooth Muscle Heavy Chain)
- 适用
- 人, 大鼠, 小鸡, 兔, 犬, 豚鼠, Pig, Cat, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Myosin (Smooth Muscle Heavy Chain) antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Myosin, Smooth Muscle Heavy Chain (ID8), Purified, with BSA
- 产品特性
- Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Human uterus extract
- 克隆位点
- ID8
- 亚型
- IgG1 kappa
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Uterus or normal breast
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Myosin (Smooth Muscle Heavy Chain)
- 别名
- Myosin, Smooth Muscle Heavy Chain (Myosin (Smooth Muscle Heavy Chain) 产品)
- 背景
- AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC
- 分子量
- 205 kDa (MHC-1) and 200 kDa (MHC-2)
- 基因ID
- 4629, 460109
- UniProt
- P35749
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