Recombinant Keratin Acidic (AE1) 抗体 (CF®594)
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- 抗原 See all Keratin Acidic (AE1) 抗体
- Keratin Acidic (AE1)
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- CF®594
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)
- 原理
- Recombinant Rabbit Monoclonal anti-Cytokeratin, Acidic (KRTL/1577R), CF594 Conjugate
- 产品特性
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant human KRT77 protein fragment
- 克隆位点
- KRTL-1577R
- 亚型
- IgG kappa
- Top Product
- Discover our top product AE1 Primary Antibody
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- 应用备注
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Immunohistology (formalin) 1-2 μg/mL
- Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Skin, Squamous cell carcinoma (SCC)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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