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Recombinant Keratin Acidic (AE1) 抗体 (CF®594)

AE1 适用: 人 IHC, IF, WB, FACS 宿主: 兔 Monoclonal KRTL-1577R CF®594 Recombinant Antibody
产品编号 ABIN6178768
发货至: 中国
  • 抗原 See all Keratin Acidic (AE1) 抗体
    Keratin Acidic (AE1)
    抗体类型
    Recombinant Antibody
    适用
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    宿主
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    克隆类型
    • 31
    单克隆
    标记
    • 15
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    CF®594
    应用范围
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    Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)
    原理
    Recombinant Rabbit Monoclonal anti-Cytokeratin, Acidic (KRTL/1577R), CF594 Conjugate
    产品特性
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Recombinant human KRT77 protein fragment
    克隆位点
    KRTL-1577R
    亚型
    IgG kappa
    Top Product
    Discover our top product AE1 Primary Antibody
  • 应用备注
    Immunohistology (formalin) 1-2 μg/mL
    • Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    说明

    Skin, Squamous cell carcinoma (SCC)

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    Keratin Acidic (AE1)
    别名
    Cytokeratin, Acidic (AE1 产品)
    背景
    K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B
    分子量
    56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19)
    基因ID
    374454, 334989
    UniProt
    Q7Z794
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