Keratin 5/8 抗体
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- 抗原 See all Keratin 5/8 (KRT5/8) products
- Keratin 5/8 (KRT5/8)
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适用
- 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 5/8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), Purified, BSA-free
- 产品特性
- This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Cytoskeletal preparation from HeLa cells
- 克隆位点
- C-50
- 亚型
- IgG1 kappa
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anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM268 unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Does not react with chicken , or Xenopus
- Optimal dilution for a specific application should be determined by user
- 说明
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HepG2 or A431 cells. Skin or Squamous cell carcinoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- Keratin 5/8 (KRT5/8)
- 别名
- Cytokeratin 5/8 (KRT5/8 产品)
- 分子量
- 58 kDa (CK5), 52.5 kDa (CK8)
- 基因ID
- 3852, 3856
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